Application | Comment | Organism |
---|---|---|
drug development | ATX is one of the target molecules for cancer therapy, therefore the immuno-neutralization by an antibody which is able to recognize the catalytic site, or the low molecular weight chemical inhibitors based on structural biology should be a useful tool for prevention or treatment of tumor progression and metastasis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 and Escherichia coli TOP10 strain. Construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | rhATX S48 site-directed mutagenesis, recombinant human ATX, creation of a full-length recombinant molecule by using prokaryotic cells. Shows lysoPLD enzymatic activity. hATX S48 Ser-48-C terminal of human ATX-T (L46720) cDNA is amplified. hATX S48 protein shows both lysoPLD and PDE activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | N-terminal transmembrane type glycoprotein | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | similar stimulation of LysoPLD activity by addition of Ca2+ and Mg2+ occurs. Mg2+ or Ca2+ may act to stabilize the structure of ATX or shield the electric charge of the substrate | Homo sapiens | |
Co2+ | activity of hATX S48 protein is significantly stimulated by addition of Co2+ | Homo sapiens | |
Mg2+ | similar stimulation of LysoPLD activity by addition of Ca2+ and Mg2+ occurs. Mg2+ or Ca2+ may act to stabilize the structure of ATX or shield the electric charge of the substrate | Homo sapiens | |
Ni2+ | Mg2+-ATP treated sample is applied to the Ni2+ charged chelating sepharose. Most effective affinity chelating metal | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
transmembrane protein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidyl choline + H2O | Homo sapiens | - |
lysophosphatidic acid + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13822 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
gel filtration, SDSPAGE. Establish purification protocol for recombinant hATX S48 protein. Introduction of the construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HT-1080 cell | Various concentrations of hATX S48 protein are exposed to HT-1080 cells and motile area is analyzed. hATX S48 protein also affects cell proliferation | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
biochemical and cell biological activity of hATX S48 protein measured | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidyl choline + H2O | - |
Homo sapiens | lysophosphatidic acid + choline | - |
? | |
lysophosphatidyl choline + H2O | various concentration of lysophosphatidyl choline with or without 2.5 pM of hATX S48 are exposed to HT-1080 cells and motile area is analyzed | Homo sapiens | lysophosphatidic acid + choline | - |
? | |
additional information | Catalytic ability of ATX for p-nitrophenyl thymidine 5'-monophosphate and adenosine triphosphate hydrolysis are low compared with nucleotide pyrophosphatases-1/phosphodiesterases | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATX | one of the well-known tumor malignant factors | Homo sapiens |
autotaxin | - |
Homo sapiens |
human autotaxin | - |
Homo sapiens |
lysophospholipase D | - |
Homo sapiens |
lysoPLD | - |
Homo sapiens |
rhATX S48 | recombinant human ATX (rhATX S48) from prokaryotic cells | Homo sapiens |